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KMID : 1098420110190010009
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Korean Journal of Medicinal Crop Science
2011 Volume.19 No. 1 p.9 ~ p.15
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Conservation of An Endangered Species of Lilium cernum Komarvo. through in vitro Mass-Propagation
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Lee Song-Hee
Lee Su-Gwang
Kang Ho-Duck
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Abstract
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This study was conducted to establish the optimal condition for conservation of genetic resources and micropropagation of Lilium cernum. Induction of bulbet of L. cernum was highly effective (9.2 bulb/explant) on 1/2 SH (Schenk and Hildebrandt) medium supplemented with 1.0 ㎎/L TDZ (Thidiazuron) and 0.1 ㎎/L NAA (Naphthaleneacetic acid). The treatment of 0.1 ㎎/L NAA increased root development (6.4 root/explant) under the in vitro condition. In addition, treatments of AC (Activated Charcoal) and ventilation were enhanced to develop number of shoots and to elongate length of leaf, bulb and root. Futhermore, the process of short-term soil acclimatization was promoted to strengthen the plantlets induced under the in vitro condition.
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KEYWORD
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Lilium cernum, in vitro, Plant Regeneration, Ventilation, Soil Acclimatization
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